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RAISING PURE WHITEFLY COLONY
Tuesday, 3rd April 2018

Bemisia tabaci is a plant sap-sucking insect in family Aleyrodidae. B. tabaci is a species complexthat cannot be distinguished morphologically. Therefore, to produce a pure colony it isimportant to raise the initial population from a single fertilized female.The adult female usually lays eggs underneath the tender leaf of the host plant as it feeds. Eggsare initially whitish and change to brown towards hatching. Eggs hatch into first nymphal instar(usually semi-transparent) known as crawler, which move short distances for about 1 3 hourslooking for a suitable place to feed and develop. After the first moult into the second instar, thenymph becomes immobile, and is oval and flattened. The process of moulting continues leadingto third and fourth instar.The second, third and fourth instars secrete a waxy material at the margins of their body thathelps adhere them to the leaf surface. The different instars subsequently increase in size andbecome more yellow, and the fourth instar has noticeable red eyes when viewed under astereo microscope. The fourth instar does not pupate but emerges from the last molt as adultleaving an exoskeleton called exuvium with a characteristic T-shaped exit hole.Freshly emerged males and females are sexually immature and have clear wings that graduallyget coated with wax. The emerged adults sexually mature during the next 24 hours. Virginfemales are able to lay eggs but offspring will be only males. Mated females will produce bothmale and female offspring

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